Question: Short And Long Read Sequencing

Question: Short And Long Read Sequencing

What is the difference between short and long read sequencing?

What are the comparative advantages of each?

Which one of them is used now-a-days for sequence assembly? and

Which one is preferred for future from both the computational and analytical point of view?

The difference is in the length of the reads :-)

The term 'short read' came about to describe technologies that produced reads that were substantially shorter (30-50bp) than the mainstream technologies employed at that point (1000bp). The 'short read' sequences are getting longer as the technologies evolve but as far as I know there is no length at which sequencing would be called 'long read'.

Short read technologies produce higher coverage. Longer reads are easier to process computationally and interpret analytically.

A trade-off between the various needs and requirements determines the right choice.

Just to add a little:

As mentioned, I believe that there is generally a trade off between length of sequence and number of reads (coverage).

Sometimes longer reads are needed. For example, to cover more than one exon/exon junction in mRNA to deduce isoforms.

Also, the way a library is prepared is usually very important for analysis. Often times an RNA sequencing experiment will remove small RNAs from a library before sequencing - microRNAs, siRNAs, and other small RNAs will not present in the sequencing results, so these libraries can't be used for projects analysing those.

问:短读和长读序列

短读序列和长读序列的区别是什么?

它们各自的比较优势是什么?

它们中的哪一个现在被用于序列装配? 和

从计算和分析的角度来看，哪一个是未来的首选?

一。区别在于读取的长度:-)

“短读取”一词的出现是为了描述产生的读取量比当时使用的主流技术(1000个bp)短很多(30-50bp)的技术。 随着技术的发展，“短读”序列变得越来越长，但据我所知，没有长度的序列可以被称为“长读”。

短读技术产生更高的覆盖率。 再读更容易计算和解释分析过程。

各种需求和需求之间的权衡决定了正确的选择。

再补充一点:

正如前面提到的,我相信总有一序列的长度之间的权衡和读取的数量(报道)。

有时需要更长的read。 例如，覆盖mRNA中不止一个外显子/外显子连接以推断异构体。

此外，库的准备方式通常对分析非常重要。 通常，RNA测序实验会在测序前从文库中移除小RNA——microRNAs、siRNAs和其他小RNA不会出现在测序结果中，所以这些文库不能用于分析这些小RNA的项目。