Advances in Single Molecule, Real-Time (SMRT) Sequencing

    科技2022-07-16  125

    Advances in Single Molecule, Real-Time (SMRT) Sequencing

    Special Issue Information

    Dear Colleagues,

    PacBio’s single molecule, real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules.

    From microbes to vertebrates, long reads are now used to create highly accurate de novo genome assemblies, characterize complex structural variations, permit full-length RNA isoform sequencing, and directly phase variants. The high accuracy further enables low frequency mutation detection and clonal evolution determination. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications.

    Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are being continuously pushed by developments in bioinformatics and sample preparation.

    This Special Issue is a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.

    Dr. Adam Ameur Dr. Matthew S. HestandGuest Editors

     

    Manuscript Submission Information

     

    Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

    Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

    Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

     

    For this Special Issue we are glad to offer a 15% discount from our APC to all planned contributions. Please contact and inform maria.noguera@mdpi.com in advance for this purpose. 

    Keywords

    SMRT sequencingPacBiogenome assemblystructural variationRNA isoformsDNA base modificationstargeted sequencingclinical sequencingbioinformatics

    亲爱的同事们,

    PacBio的单分子实时(SMRT)测序技术与目前主导市场的短读DNA测序技术相比具有重要优势。 这包括异常长的读取长度(20kb或更多),无与伦比的一致性准确性,以及对固有的、非扩增的DNA分子进行排序的能力。 从微生物到脊椎动物,长读谱现在被用于创建高精度的从头基因组装配,表征复杂的结构变异,允许全长RNA亚型测序,和直接阶段变异。 高精度进一步实现了低频突变检测和克隆进化测定。 除了减少偏倚,测序原生DNA也允许直接测量DNA碱基修饰。 因此,SMRT测序在农业、基础科学和医学研究等许多领域已成为一项具有吸引力的技术。 生物信息学和样品制备的发展不断推动着SMRT测序的边界。

    本期特刊是一系列展示SMRT测序技术的最新发展和应用的广度的文章。

    亚当Ameur博士 Matthew S. Hestand博士 客人编辑

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    提交的手稿以前不应发表,也不应考虑在其他地方发表(会议论文集除外)。 所有的稿件都是通过单盲同行评审过程进行彻底的评审。 作者指南和其他相关信息提交的手稿可在作者指南页。 基因是一个国际同行评议MDPI每月开放获取期刊发表。

    请在提交手稿前访问作者说明页。 在本开放存取期刊上发表的文章处理费为1800瑞士法郎。 提交的论文应格式正确,并使用良好的英语。 作者可以使用MDPI的英文编辑服务在出版前或在作者修改期间。

    这个特殊的问题我们很高兴提供15%的折扣从我们APC所有计划的贡献。 为此,请提前联系并告知maria.noguera@mdpi.com。

    关键字 SMRT测序  PacBio      基因组组装   结构变化   RNA亚型   DNA基础修改  有针对性的测序    临床测序   生物信息学

    参考文献:https://www.mdpi.com/journal/genes/special_issues/SMRT_sequencing

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