Dear Colleagues,
PacBio’s single molecule, real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules.
From microbes to vertebrates, long reads are now used to create highly accurate de novo genome assemblies, characterize complex structural variations, permit full-length RNA isoform sequencing, and directly phase variants. The high accuracy further enables low frequency mutation detection and clonal evolution determination. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications.
Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are being continuously pushed by developments in bioinformatics and sample preparation.
This Special Issue is a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.
Dr. Adam Ameur Dr. Matthew S. HestandGuest Editors
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亲爱的同事们,
PacBio的单分子实时(SMRT)测序技术与目前主导市场的短读DNA测序技术相比具有重要优势。 这包括异常长的读取长度(20kb或更多)，无与伦比的一致性准确性，以及对固有的、非扩增的DNA分子进行排序的能力。 从微生物到脊椎动物，长读谱现在被用于创建高精度的从头基因组装配，表征复杂的结构变异，允许全长RNA亚型测序，和直接阶段变异。 高精度进一步实现了低频突变检测和克隆进化测定。 除了减少偏倚，测序原生DNA也允许直接测量DNA碱基修饰。 因此，SMRT测序在农业、基础科学和医学研究等许多领域已成为一项具有吸引力的技术。 生物信息学和样品制备的发展不断推动着SMRT测序的边界。
本期特刊是一系列展示SMRT测序技术的最新发展和应用的广度的文章。
亚当Ameur博士 Matthew S. Hestand博士 客人编辑
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关键字 SMRT测序 PacBio 基因组组装 结构变化 RNA亚型 DNA基础修改 有针对性的测序 临床测序 生物信息学
参考文献：https://www.mdpi.com/journal/genes/special_issues/SMRT_sequencing
